Assessment of the phylogenetic relationships within the spondylidine branch of Spondylidinae (Coleoptera, Cerambycidae)

Abstract Spondylidinae is a minor subfamily of Cerambycidae with around 100 species in 20 genera. The tribes Asemini and Spondylidini, both salient owing to vectors invaders into the limelight phytosanitary authorities, constitute “spondylidine branch”. Although few branch were being included some higher taxonomic level studies, phylogenetic relationships within taxa have never been evaluated from molecular viewpoint. present study identifies inferring single locus ( COI ) global dataset two multi-locus local datasets, binary combinations mitochondrial , 16S rRNA nuclear 28S D1-D2 that sequences obtained samples East Marmara Region, Turkey. [( Spondylis+Neospondylis + Megasemum ] [ Tetropium+Asemum groups separated same node trees. genus Cephalallus was stated on basalmost these groups. Arhopalus split all other genera divided subgroups. There discrepancies between morphology barcode sequences, which might be sign hybridisation A. rusticus syriacus . Intraspecific distances gene regions S. buprestoides As. striatum T. cinnamopterum up 4.26%, 8.51%, 6%, respectively, point out cryptic speciation.